iTRAQ ( Isobaric Tags for Relative and Absolute Quantitation) 技术是ABI公司推出的一项新的体外同位素标记技术。使用该技术可以对一个基因组表达的全部蛋白质或一个复杂的混合体系中的所有蛋白质进行精确定量和鉴定。寻找差异表达蛋白，并揭示其细胞生理病理功能。联川生物依托先进的蛋白质谱分析平台、强大的信息分析和数据处理能力，为用户提供高效的蛋白质组鉴定与定量分析服务。
1.Wang L，et al. Proteome profiling reveals immune responses in Japanese flounder (Paralichthys olivaceus) infected with Edwardsiella tarda by iTRAQ analysis. Fish Shellfish Immunol. 2017 Jul;66:325-333. doi: 10.1016/j.fsi.2017.05.022
2.Zhang G, Zhang J, Wen X, et al. Comparative iTRAQ-based quantitative proteomic analysis of Pelteobagrus vachelli liver against acute hypoxia: Implications in metabolic responses.[J]. Proteomics, 2017.
3.Li R, Yu H, Yue Y, et al. Combined proteomics and transcriptomics identifies sting-related toxins of jellyfish Cyanea nozakii.[J]. Journal of Proteomics, 2016, 148:57.
4.Lv LX, et al.(2016) Integrated transcriptomic and proteomic analysis of the bile stress response in probiotic Lactobacillus salivarius LI01. Journal of Proteomics. doi.org/10.1016/j.jprot.2016.08.021
5.Lv LX, et al.(2016) Integrated transcriptomic and proteomic analysis of the bile stress response in probiotic Lactobacillus salivarius LI01. Journal of Proteomics. doi.org/10.1016/j.jprot.2016.08.021
6.Wang C, Liu C, Wei L, Shi L, Pan Z, Mao L, Wan X, Ping Z, Jiang T, Chen Z. (2016) A Group of Novel Serum Diagnostic Biomarkers for Multidrug-Resistant Tuberculosis by iTRAQ-2D LC-MS/MS and Solexa Sequencing. International Journal of Biological Sciences 12(2), 246-256.
7.Cui Y, et al. (2016) Transcriptomic/proteomic identification of allergens in the mite Tyrophagus putrescentiae. Allergy.DOI:10.1111/all.12999
8.Cao JY, et al. (2016) TMT-based quantitative proteomics analyses reveal novel defense mechanisms of Brassica napus against the devastating necrotrophic pathogen Sclerotinia sclerotiorum. J Proteomics. doi: 10.1016/j.jprot
KOG/COG注音
COG（Clusters of Orthologous Groups of proteins），即同源蛋白簇。一般原核 生物用COG，真核生物用KOG。COG注释作用：1. 通过已知蛋白对未知序列进行功能注释；2. 通过查看指定的COG编号对应的protein数目，存在及缺失，从而能推导特定的代谢途径是否存在； 3. 每个COG编号是一类蛋白，将query序列和比对上的COG编号的proteins 进行多序列比对，能确定保守位点，分析其进化关系。
Lv LX, et al.(2016) Integrated transcriptomic and proteomic analysis of the bile stress response in probiotic Lactobacillus salivarius LI01.Journal of Proteomics. doi.org/10.1016/j.jprot.2016.08.021
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